In-vivo Inhibition of Glutathione S-Transferase P1 by Bovine Lactoferricin in Swiss Albino Tumor Bearing Mice

Article history: Received on: 23/09/2016 Revised on: 28/10/2016 Accepted on: 10/11/2016 Available online: 29/11/2016 Many cancers overexpress GSTP1 which contributes to multidrug resistance via conjugation of antineoplastic drugs. The aim of this paper is to investigate the in-vivo interaction of GSTP1 and Lfcin B in Swiss albino female mice induced with skin cancer using 7, 12 dimethylbenz (a) anthracene. Group B and C received topical application of DMBA, whereas group C was subcutaneously injected with 200μl (20μg/μl) Lfcin B while keeping group A as a control. The activity of GSTP1 in all the groups was then estimated spectrophotometrically at 340nm by taking the increase in absorbance of GS-DNB conjugate. Administration of 200μl (20μg/μl) Lfcin B three times per week was found to significantly (p<0.01) inhibit GSTP1. GSTP1 was observed to be overexpressed in group B (treated with DMBA only as skin carcinogen). Significant differences (P<0.01) were observed between group A (control) and C (DMBA+ Lfcin B group) indicating in vivo inhibition of GSTP1 by Lfcin B. We concluded that Lfcin B competitively inhibits GSTP1 in-vivo. Accordingly, Lfcin B may emerge as a suitable pharmacological agent that can selectively target GSTP1 thereby inducing cancer cells to apoptosis.